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Laboratory Manual for GENETIC ENGINEERING: Book by John Vennison

Laboratory Manual for GENETIC ENGINEERING

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ISBN: 9788120338142    Publisher: PHI LEARNING PVT. LTD. Year of publishing: 2009     Format:  Paperback No of Pages: 140        Language: English
This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering.

The book explains the methods...Read more

This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering.

The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens.

This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology.

KEY FEATURES :

Includes about 60 different experiments.

Contains several figures to reinforce the understanding of the techniques discussed.

Gives useful information about preparation of stock solutions, DNA/protein conversions, restriction enzymes and their recognition sequences, and so on in Appendices.
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About the author: John Vennison
S. John Vennison, Ph.D., is Lecturer in the Department of Biotechnology, Anna University, Tiruchirappalli. He has more than 10 years of teaching experience... Read more
S. John Vennison, Ph.D., is Lecturer in the Department of Biotechnology, Anna University, Tiruchirappalli. He has more than 10 years of teaching experience and 18 years of research experience in the field of genetic engineering. He has published several research papers in the national and international journals. His research areas include molecular biology and biotechnology. A recipient of the Visiting Scientist Award, 1995 by UNESCO, Dr. Vennison is a lifemember of Biotech Research Society of India.
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